Plasma albumin determination on the Cobas Bio centrifugal analyser using bromocresol green

Determination of free haemoglobin in serum by an automated assay using 4-aminophenazone and the Cobas Bio System.

A colorimetric method for the determination of free haemoglobin in human serum is described, using 4-aminophenazone as chromogen, and commercially available haemiglobin cyanide solutions as standards. The reaction system is suitable for manual measurements as well as determinations with a centrifugal analyser (Cobas Bio System). Accuracy, linearity and stability of the procedure is shown. A ref...

Immunoturbidimetric assays for serum apolipoproteins A1 and B using Cobas Bio centrifugal analyser.

Immunoturbidimetric assays for measuring the apolipoproteins A1 and B using the Cobas Bio centrifugal analyser are described. The methods were specific, offered good sensitivity (less than 0.05 g/l) and intrabatch variability, with coefficients of variation between 2.4% and 3.5%, and were cost effective. Reference ranges were calculated for a group of civil servants, aged 35 to 55 years.

Evaluation of two kinetic methods for serum amylase using a Cobas Bio centrifugal analyser

Bromocresol purple, a non-specific colour reagent for the determination of serum albumin.

The determination of serum albumin with the dye reagent bromocresol purple was investigated. We found that bromocresol purple is not a specific reagent for albumin, but that serum proteins in the alpha-, beta-, and gamma-globulin fractions also react with this dye. Similar results were obtained for bromocresol green. Furthermore there is a great difference in absorbance between human and bovine...

The introduction of bromocresol purple for the determination of serum albumin on SMAC and ACA, and the standardization procedure.

The bromocresol purple methods for the determination of serum albumin on the Technicon SMAC and the DuPont ACA systems were evaluated and compared with the corresponding bromocresol green methods, a radial immunodiffusion (RID) and an electrophoresis technique. The linearity and precision of the dye binding methods were comparable. The bromocresol purple methods were more influenced by lipaemia...